Aqua Natural Oolitic Aragonite Bahamas sand is raw, naturally renewable Oolitic Aragonite sand that is up to 98% pure calcium carbonate. The grains are OOLITIC (egg-shaped) and smooth. It is tasteless, odorless, dustless and sustainable. Combined with its near chemical purity, size consistency, and small grain structure, Bahamas aragonite possesses numerous advantages making it a superior source of calcium carbonate. Contains a patented blend of live nitrosomas and nitrobacter bacteria which break down and convert harmful ammonia, into nitrates. Directions for use: PRIMARY (not mixed with Dry Aragonite) Usage Calculator: Use 1lb (450g) of Bio-Substrate for every 1 gallons (3.8 litres) of water. - DO NOT rinse before use. - Spread Aragonite and fill with water slowly. Avoid stirring up the substrate. - Once the tank is filled, start your filter. Any cloudy appearance should clear over time. MIXED (mixed with Dry Aragonite) Usage Calculator: Use 1lb (450g) of mixed LIVE Bio-Substrate and DRY Substrate for every 1 gallons (3.8 litres) of water. Mixing ratio is 1:4 (1 LIVE to 4 DRY). Directions for use: - DO NOT rinse before use. - Mix Bio-Substrate and Dry Aragonite thoroughly. - Now spread mixed Aragonite and fill with water slowly. Avoid stirring up the substrate. - Once the tank is filled, start your filter. Any cloudy appearance should clear over time. LIVE nitrosomas and nitrobacter bacteria are harmless to marine life. The live nitrifying bacteria will immediately get to work forming micro colonies of beneficial ammonia busting bacteria. Use with any corresponding Aqua Natural Dry Aragonite: - Oolitic Aragonite - Coarse Aragonite - African Cichlid